This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation.One of the proteins is overexpressed in E.coli as HA-tagged recombinant protein and cell-free extracts are immunoprecipitated in HA-affinity resin.Cell extracts are treated with nuclease to degrade DNA and RNA, which rules out nucleic
Understanding the relationship between communities and wetland ecosystems is imperative to facilitate the development of wetland management and conservation strategies that can effectively safeguard wetland health and sustain the flow of ecosystem services.To understand the knowledge, attitude, and perception of communities on wetland ecosystem ser
Abstract Background The discourse about mental health problems among Theft Recovery migrants and refugees tends to focus on adverse pre-migration experiences; there is less investigation of the environmental conditions in which refugee migrants live, and the contrasts between these situations in different countries.This cross-national study of two
Problem.Educational work is an integral part of the activities of teachers of higher education, aimed at inculcating a culture of work and professional activities; uniting the scientific work of students and educational activities; formation of ecological culture, healthy lifestyle and habits of safe living.Goal is the exchange of experience in imp
The setting of the late poured band is an effective measure to control the differential settlement and the stress concentration of the foundation.However, the lag of the pouring time of the late poured band will also bring adverse effects to the construction process.Taking a large commercial complex as an Curling - Clothing - Womens example, Midas